Medium to facilitate the growth of Lactobacilli in general.

FORMULA IN GRAMS PER LITER

Final pH 6.2 ± 0.2 at 25ºC

Uses
MRS BROTH has the same formulation as MRS Agar (Cat. BIOAMRSABA500) without the agar as a solidifying agent. It is used when a fluid medium is preferred. It was developed by de Man, Rogossa and Sharpe to provide a medium that would support the good growth of Lactobacilli in general, but in particular for those strains which showed poor growth in existing media such as L. brevis and L. fermenti, replacing a variable product (tomato juice).
The medium is apt for the growth of lactic acid bacteria, including Lactobacillus, Streptococcus, Pediococcus and Leuconostoc. Ammonium citrate at a low pH inhibits most microorganisms, including other Streptococci and molds and limits swarming, but allows for the growth of Lactobacilli. Dipotassium phosphate and Sodium acetate are buffer agents to maintain a low pH; Tween 80 is an emulsifier; Manganese and Magnesium sulfates are sources of ions and sulfate; Peptone, Beef and Yeast extracts are nutrient sources for growth: nitrogen, vitamins, minerals and amino acids. Dextrose is the fermentable carbohydrate for carbon and energy source. The times and temperatures of incubation are the same as in MRS Agar (35 ± 2°C for 3 days or better, 30°C for 5 days). Tubes showing growth are subcultured to MRS Agar to confirm the presence of Lactobacilli. MRS Broth may be used for other tests in the identification of Lactobacilli, such as temperature dependence, growth in 4% NaCl, growth in 0.4% Teepol, etc. as recommended by Sharpe, Fryer and Smith.

Size:
500g

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Mrs Broth