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Real HotStart DNA Polymerase Mastermix is an ultra-sensitive PCR product. It works excellent especially for short DNA template (size shorter than 600 bp). It contains all the factors and loading dye* needed to perform PCR, including HotStart DNA polymerase, dNTPs, 7.5 mM MgCl2 , loading dye*(including bromphenol). The only step to perform PCR is to add the template and primers into the tube containing RealStart DNA Polymerase Mastermix. Since the special HotStart DNA polymerase in Real HotStart DNA Polymerase Mastermix activates only after heating, it also reduces the risk of contamination when working with Real HotStart DNA Polymerase Mastermix at room temperature. Real HotStart DNA Polymerase Mastermix makes PCR simple and easy, eliminating the extra handling steps and contamination risks associated with conventional hot-start methods.
Contents:
250 units. with loading dye
Reaction: 100 Reactions
2X Real HotStart DNA Polymerase Mastermix
(200 U/ml): 1. 25 ml
Control DNA Template (1x10-14g) : 3 µl
Control Primer Mix (5 µM) : 4.5 µl
Sterile Deionized Water : 1.5 ml
Shipment condition: -20°C
Storage condition: -20°C
Note: For research use only. Not for use in diagnostic or therapeutic procedures.
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